Regulatory

Part:BBa_K4628018:Design

Designed by: Linghao Xia   Group: iGEM23_ZJU-China   (2023-10-11)


subPromoter CP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By mutating the start codon of CP protein coding sequence within promoter sequence, we successfully used the modified promoter to express GFP that can emit light correctly


Source

Tobacco mosaic virus genome (NCBI Reference Sequence: NC_001367.1)

References